2023, Vol. 3, Issue 2, Part A
A study on therapeutic drug monitoring (TDM) of 5-fluorouracil
Author(s): Malkhedkar Aditya Aniruddha and Dr. Anuj Kumar Sharma
Abstract: Finalizing an appropriate analytical method for identifying and quantifying pharmaceuticals and their metabolites in various bodily fluids is critical throughout method development. For the estimate of pharmaceuticals and their metabolites in biological fluid, the detection technology chosen should be very sensitive, precise, and accurate. The sample preparation method chosen should provide consistency in recovery and repeatability, as well as minimal or no interference with substances of interest during instrumental analysis. Different extraction methodologies such as protein precipitation extraction, liquid-liquid extraction, and solid phase extraction are evaluated based on the nature of the biological matrix and drug's physico-chemical properties such as molecular structure, polarity, partition coefficient, solubility, and dissociation constant, among others, to extract neat form of the compounds of interest free from endogenous substances such as proteins, phospholipids, sugars, and salts present in the biolo During mass spectrometric analysis, endogenous molecules such as phospholipids and proteins typically cause ion suppression, resulting in incorrect results. Furthermore, adjusting chromatographic settings is critical for maximum throughput, selectivity, and sensitivity when testing substances of interest. Flow rate, column, column temperature, mobile phase combination, and reconstitution solution are all variables that must be optimized. If any of the above parameters are not appropriately optimized, the analysis will suffer, resulting in poor sensitivity and uneven recoveries, and the method will need to be redeveloped. As a result, the technique development process is critical in bioanalysis.
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How to cite this article:
Malkhedkar Aditya Aniruddha, Dr. Anuj Kumar Sharma. A study on therapeutic drug monitoring (TDM) of 5-fluorouracil. Int J Pharm Sci Drug Anal 2023;3(2):56-59.