Author(s): Halla Wahab Razooqi Alasadi, Selman Mohammed Selman and Rana A Ghaleb

Abstract: Background: Colon cancer, which affects both men and women, is the second most lethal kind of cancer and a global health concern. The world's colon cancer death rate is rising as a result of the high meat and alcohol consumption of modern diets and lifestyles, combined with little physical exercise. Therefore, the development of innovative and ecologically safe pharmacological therapy for colon cancer is necessary. Nutraceuticals are now being used to treat a number of chronic conditions, including diabetes, Alzheimer's disease, and colon cancer. Nutraceuticals come from a variety of natural sources, including fruits, vegetables, marine life, and medicinal plants. It has been demonstrated that nutraceuticals may both lower the risk and delay the development of colon cancer. These food ingredients focus on several molecular elements of colon cancer growth. Building on its traditional therapeutic applications, researchers are looking into natural substances with possible anticancer effects, such as the latex of Tamarix mannifera. This study investigates the apoptotic effects of Tamarix mannifera alone and in conjunction with traditional anticancer medications and chemotherapeutic agents on the LS174T cell line. By comprehending these connections, potentially safer and more effective therapies for colon cancer may be discovered.Method: For 24 hours, the human colon cancer LS174T cell-line was given a variety of doses of cisplatin, doxorubicin and Tamarix mannifera each alone and their combinations. MTT assay was used to assess the cytotoxicity of the agents, then the supernatant was used to measure the level of caspase 3 level. Results: Tamarix mannifera significantly increase cytotoxicity (P-value ≤0.05) at concentrations from 31.25 to 1000 μg/ml, with the most notable increase in cytotoxicity at 1000 compared to 125 μg/ml. Cisplatin also increase cytotoxicity at concentrations from 7.8125 to 250 μg/ml, most notably at 250 compared to 15.625 μg/ml. doxorubicin also increase cytotoxicity at concentrations from 7.81 to 250 μg/ml, most notably at 250 compared to 31.25 μg/ml. Combining Tamarix mannifera with cisplatin increase cytotoxicity at various concentrations but showed significant increase cytotoxicity (P-value ≤0.05) at 250 compared to 125 μg/ml. also combining Tamarix mannifera with doxorubicin increase cytotoxicity at various concentrations but showed significant increase cytotoxicity (P-value ≤0.05) at 31.25 compared to 500 μg/ml.Conclusion: Tamarix mannifera extract with cisplatin and doxorubicin increase cytotoxicity level(decrease viability of cancer cells), showing potential. However, saturation effects at greater doses imply dosage complexity. Cisplatin's effectiveness, doxorubicin's effectiveness and Tamarix 's potential anti-cancer qualities demand additional research and clinical testing.

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