International Journal of Pharmaceutical Sciences and Drug Analysis
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P-ISSN: 2788-9246, E-ISSN: 2788-9254
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2024, Vol. 4, Issue 2, Part A

Exploring the Akshya-Juned qualitative technique for carbohydrate detection in protein solutions


Author(s): Akshya Kumar Mishra and Mohammad Juned Sumra

Abstract: The Akshya-Juned Qualitative Technique is a novel method for detecting carbohydrates in protein solutions, utilizing a rapid colorimetric approach. This technique involves treating protein samples, including milk, albumin, and albumin mixed with dextrose, with 40% sodium hydroxide (NaOH) and sodium acetate. The alkaline environment facilitates the hydrolysis of proteins and the breakdown of lactose into simpler sugars, promoting the Maillard reaction between amino acids and reducing sugars. This reaction produces melanoidins, resulting in a chocolate brown color that signifies the presence of carbohydrates. The method's effectiveness was demonstrated through positive results with milk and albumin containing dextrose, and a negative result with plain albumin. Additionally, we propose a quantitative extension of the test, where the intensity of the color change correlates with the carbohydrate content, offering a semi-quantitative measure of carbohydrates in protein-rich samples. This technique provides a straightforward, efficient means of carbohydrate detection, with potential applications in biochemical analysis and quality control.

Pages: 10-11 | Views: 27 | Downloads: 15

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International Journal of Pharmaceutical Sciences and Drug Analysis
How to cite this article:
Akshya Kumar Mishra, Mohammad Juned Sumra. Exploring the Akshya-Juned qualitative technique for carbohydrate detection in protein solutions. Int J Pharm Sci Drug Anal 2024;4(2):10-11.
International Journal of Pharmaceutical Sciences and Drug Analysis
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