Author(s): Sonal Balasaheb Bangar, Soham Sunil Lad, Sakshi Sunil Gaikwad, Abhishek Sanjay Masal, Parag Ganesh Bayaskar, Dnyaneshwari Bappa Misal and Shrutika Patil

Abstract: This research focuses on the formulation and assessment of an innovative facial serum incorporating anthocyanins naturally occurring polyphenolic compounds sourced from the vivid petals of Clitoria ternatea (butterfly pea flower). Anthocyanins are a class of flavonoids responsible for the vibrant blue to purple pigmentation observed in many plants, and they are increasingly recognized for their potent biological activities, particularly their exceptional antioxidant capabilities. In this formulation, the serum is specifically designed to harness these properties, aiming to deliver comprehensive skincare benefits through natural bioactivity. Anthocyanins are powerful natural compounds that can give away hydrogen atoms or electrons, which helps neutralize harmful molecules called free radicals and reactive oxygen species (ROS). These harmful molecules cause oxidative stress, a major reason for skin aging. By fighting them, anthocyanins help reduce fine lines and wrinkles, improve skin tone and firmness, and make the skin stronger overall. In this study, anthocyanins were carefully taken out from plants using a method that uses acidified methanol to keep them stable and active. To keep them from breaking down since they are sensitive to pH changes, they were mixed into the serum's water base with natural stabilizers and pH buffers. Antioxidant capacity of the serum was rigorously evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) radical scavenging assay, where the serum exhibited a free radical scavenging activity exceeding 85%. By providing strong protection against oxidative stress and promoting overall skin health, the developed serum represents an innovative, biocompatible alternative to synthetic antioxidant agents, aligning with the growing consumer demand for plant-based and efficacious cosmetic products.

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