2025, Vol. 5, Issue 2, Part A

Novelty: The present study reports, for the first time, the development of three simple, rapid, and eco-friendly UV spectrophotometric methods for the quantitative estimation of Inosine Pranobex using distilled water as solvent and cost-effective analytical method for routine quality control, as no UV spectrophotometric methods have been previously documented.

Materials and Methods: Three methods Zero-order (259 nm), First-order derivative (213 nm), and Area under the Curve (269-249 nm) were developed and validated in accordance with ICH guidelines. Linearity was established within 2-10 μg/ml and 10-50 μg/ml concentration ranges.

Results and Discussion: All methods exhibited excellent linearity, accuracy, and precision (% RSD < 2%), with satisfactory LOD and LOQ values. Forced degradation studies confirmed the drug’s stability under various stress conditions.

Conclusion: The developed methods are novel, accurate, precise, sensitive, and economical, making them highly suitable for routine analysis of Inosine Pranobex in bulk and pharmaceutical formulations.