2025, Vol. 5, Issue 2, Part B

Objective: The present study aimed to develop and validate a simple, cost-effective, and reliable UV-spectrophotometric method for the quantification of Tedizolid phosphate in bulk and tablet formulations, in accordance with ICH guidelines.

Methods: Quantification was performed using a UV-spectrophotometer with distilled water as solvent. Tedizolid phosphate showed maximum absorbance at 299 nm (zero order) and 303 nm-293 nm (area under the curve method). The linearity range was 5–25 µg/ml, with correlation coefficients (r²) of 0.9998 and 0.9998 for zero and area under the curve method, respectively.

Results: All validation parameters-linearity, accuracy, precision, LOD, LOQ, and recovery—were within acceptable limits, confirming the reliability of the developed methods for both zero and area under the curve method.

Conclusion: The developed UV-spectrophotometric techniques provide a straightforward, reliable, and cost-effective approach for the routine quantitative determination of Tedizolid phosphate in tablet formulations.